Characterisation of New Material Based on 20% AgNCls/PMAA for Arresting Caries-affected Dentine

MARIA BELEN CABALEN; GUSTAVO FABIAN MOLINA; BURROW, MICHAEL F.

Journal of International Oral Health

Journal of International Oral Health

Año: 2023 vol. 15

0976-7428

Objectives: To test a novel non-restorative treatment method for arresting dentin caries based on silver nanoclusters (AgNCls) synthesized in polymethacrylic acid (PMAA).Methods: Synthesis of AgNCls was performed by photoreduction of AgNO3 in PMAA with 355 nm/wavelength light. Cytotoxicity was assessed using the MTT assay; antibacterial effect was determined for minimum inhibitory concentration (CIM), minimum bactericidal concentration (MBC) and colony forming (CFU) of Streptococcus mutans (S. Mutans) and Lactobacillus acidophilus (L. Acidophilus); dentine color changes were measured using a spectrophotometer; surface hardness was measured using Vickers hardness test. Evaluation of the shear bond strength (SBS) of a glass ionomer (GIC) to demineralized dentine was undertaken. Silver ion penetration into dentine was traced using SEM-EDX and Laser Induced Breakdown Spectroscopy (LIBS). 38% Silver Diammine Fluoride (SDF) was the control. Specific statistical analyses were carried out for each test.Results: Cell viability of 92-89% was found after 24-72 hours respectively. MIC and MBC were determined from 1.25% and 2.5% dilutions, respectively. Log CFU counts in 1.25% concentration were significantly lower than in the control groups. Demineralised dentine surfaces treated with AgNCls/PMAA presented color stability and the highest microhardness values. The highest SBS values of GICs were also reported for this treatment group. Presence of silver was detected constantly throughout the whole depth of the artificial lesions.