Green Microbiology Clean Production in Pharmaceutical Biotecnology

MORENO MARÍA JULIETA; CABRERA CARLA; GÓNZALEZ EXEQUIEL; DI TOTO BLESSING LILIAN; CERUSICO NICOLÁS; CHAVEZ JARA ROMINA; LAZARTE MARÍA DE LOS ÁNGELES; MALDONADO MARCOS JAVIER; RAMOS VERNIERI ALBERTO NICOLÁS; SESTO CABRAL MARÍA EUGENIA

Rosario

Congreso; IV Reunión Internacional de Ciencias Farmacéuticas; 2016

CONICET-UNR-UNC

Wastes generated in the bioprocess involved in pharmaceutical biotechnology are susceptible to be reused in other industrial processes as raw material. LEFyBiFa produces biological medicinal product (LAPS) using lactic acid bacteria (LAB) as bioreactors. This work is based on the philosophy of Cleaner Production (CP), re-using residual bacterial cells (RBC) from the process as a component of culture media to replace protein and carbohydrates sources. The objective was to reuse wastes resulting from the production line to generate products with a high added value that contribute to scientific knowledge in the field of processes in Pharmaceutical Biotechnology.The critical points of bacterial biomass generation process were established on the production line of LAPS. Biomass underwent five physical lysis methods. To determine the effectiveness of RBC lysate, proteins were quantitated using bovine albumin as a standard and FT-IR spectra were performed on solid media. 15 growth media were prepared replacing protein sources of commercial medium by RBC. It was evaluated the growth of a pool of BAL in the culture media compared to the growth in commercial medium.The biomass yield per production batch: (885.5 ± 5) mg/L. Lysate methods: sonication, cooling to -20ºC and moist heat at 1 atm overpressure, with and without added water. The maximum concentration of proteins after treatment was: (222.74 ± 2.8) mg / mL, corresponding to the method of wet heat + water. The 15 media designed showed equal or greater growth of all tested BAL. A method for quality control using FT-IR was designed.Culture media designed are useful for the growth of bacteria with high nutritional requirements and could be reintroduced to the production line.