EFFECT OF CANNABIDIOL (CBD) ON NORMAL AND HEAT-MODIFIED RED BLOOD CELLS (RBCs)

TABORDA GOMEZ, CLAUDIA; MOSCOVICZ, FRANCO; PIOVAROLI, FLORENCIA; LAZAROWSKI, ALBERTO; AUZMENDI, JERÓNIMO

Buenos Aires

Congreso; Reunión Conjunta SAIC. SAI. AAFE. NANOMED.AR; 2021

SOCIEDAD ARGENTINA DE INVESTIGACION CLINICA

In recent years, cannabidiol (CBD), a cannabinoid compound has emerged as a candidate for the control of different pathologies since it does not have a hallucinogenic effect. Several beneficial effects have been reported in pain, epilepsy, arthritis, inflammation, etc. but the mechanism by which CBD acts remains still in controversia. Also the beneficial effect, several side effects were observed in patients under CBD treatment. Among other side effects, anemia and elevation of transaminases were observed in 20-30% of the patients with refractory epilepsy under CBD treatment. We hypothesized that CBD treatment could promote anemia reducing the RBCs viability. To test this hypothesis, we use human RBCs obtained from samples with normal hematometric parameters. The CBD effect was assayed on normal or heat-damaged RBCs (hdRBCs). RBCs were exposed to an increase of temperatures (40-55ºC) and then a morphological analysis was performed on the frotis of each sample stained with Giemsa. Representative images were acquired at 100x magnification and 400-500 RBCs per frotis were analyzed using the Sharp Descriptor plugin for the free software of the National Institute of Health (NIH) ImageJ. RBCs and hdRBCs were incubated with CBD 10-5M and osmotic fragility, hemolysis and morphological characteristics were analyzed. All experiments were performed by triplication. The increase in temperature induced an increase in the mean circularity (0.82±0.04 vs 1.08±0.09, 40ºCvs55ºC, p