The effect of freeze-drying on the nutrient, polyphenol and oxidant levels of breast milk

CORTEZ, MV; SORIA, EA

Breastfeeding Medicine

Mary Ann Liebert Inc. Publishers

Año: 2016 vol. 11 p. 551 - 554

1556-8253

Objectives: Given that human milk banks need to prolong milk alimentary aptness to promote breastfeeding, technological advances are required. Thus, the aim was to establish freeze-drying capacity to conserve milk properties without further oxidative deterioration. Methods: 116 healthy women from Cordoba City (Argentina) participated. Firstly, proteins, glucose, triglycerides, polyphenols and markers (nitrites, superoxide anion, hydroperoxides, lipoperoxides, and γ-glutamyltranspeptidase) were measured in their fresh milk. Then, samples were separated into three aliquots to be treated: freezing and conservation for 6 months at -80°C (F: positive control); freeze-drying for 24 hours at ≤-70°C and ≤1.33 Pa, and conservation for 6 months at 4°C (FD: treatment of interest); freeze-drying for 24 hours at ≤-70°C and ≤1.33 Pa, and conservation for 6 months at -80°C (FD+F). Next, analyses were repeated and compared by ANOVA and Tukey test. Results: Fresh milks showed these values per L: proteins: 12.62±2.51 g, glucose: 4.44±0.25 g, triglycerides: 34.26±0.59 g, polyphenols: 53.27±8.67 mg, nitrites: 62.40±19.09 mg, superoxide: 3721.02±198.80 OD, hydroperoxides: 7343.76±294.53 OD, lipoperoxides: 7349.72±398.72 OD, γ-glutamyltranspeptidase: 4.66±0.55 IU. Glucose was decreased after F treatment (p