Accelerated aging in chia nutlets (Salvia hispanica l.) for the evaluation of deterioration by ATR-FTIR and DSC

RODRIGUEZ, MARIA EMILIA; DELADINO, LORENA; SCHNEIDER-TEIXEIRA, ALINE; IXTAINA, VANESA

Valencia

Conferencia; V International Conference la ValSe-Food and VIII Symposium Chia-Link; 2023

IA ValSe-Food Network y Chia-Link

Seed deterioration is assessed by germination and vigor tests, which require a certain sample amount and operating times. Differential scanning calorimetry (DSC) and infrared spectroscopy (FTIR) are interesting tools for this study type because they do not require too much sample, are non-destructive, and consume little experimental time. It?s necessary to find correlations between conventional and biophysical methods to apply these biophysical tools for germplasm monitoring. This study deals with the physiological and biophysical characteristics of two chia varieties (WN and MN). The nutlets were aged for 56 d at 99% of relative humidity, and 41 °C, monitoring every 7 d. The germination test was conducted at 25 °C, 12 h light and 4 repetitions of 25 nutlets. The number of germinated nutlets (GN) and the mean germination time (MGT) were determined. Spectral images were analyzed by ATR-FTIR and the thermal behavior was studied by DSC at t=0 and t=56 d. After 47 d, GN decreased significantly from 100 to 57 and from 98 to 3, and TMG increased significantly from 1.01 to 2.44 d and from 1.03 to 3 d for WN and MN, respectively. PCA separated treatments into two clusters. The 92% and 88% of the variance were explained by two components for WN and MN, respectively. The fusion enthalpy increased from 15.86 to 18.87 and from 17.54 to 19.06 J/g for WN and MN, respectively, negatively correlating with GN and positively with TMG. Unconventional techniques for the rapid study of physiological quality are promising.