Cytoprotective role of Humanin against oxidative stress in granulosa cells

JULIA GAETANA CONTE; MARÍA SOL GOSSO; MERCEDES IMSEN; ADRIANA SEILICOVICH; MARINA PELUFFO; GABRIELA ALEJANDRA JAITA

Mar del Plata, Buenos Aires

Congreso; Reunión Anual de Biociencia 2019; 2019

SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC)

Humanin (HN) exerts a cytoprotective action in the presence of pro-oxidative agents in several tissues. Previously, we reported that inhibition of endogenous HN increases apoptosis in the human granulosa-like tumor cell line (KGN). Also, we demonstrated that HN decreases ROS production in KGN cells exposed to pro-oxidative environment. In the present study, our aim was to evaluate the anti-apoptotic action of HN, as a cytoprotective mechanism against oxidative stress in granulosa cells. To explore this aim, we assessed the percentage of TUNEL positive KGN cells incubated with HN (1 µM) for 30 min and H2O2 (150 µM) for further 1 h. Our results demonstrated that HN decreased the percentage of KGN TUNEL-positive cells induced by H202 (C: 0.3, HN: 0.7, H202: 2.1, H202+HN: 0.7, *p