DIETARY SUPPLEMENTATION WITH INTEGRAL CHIA AND FLAX FLOURS AMELIORATES SYSTEMIC INFLAMMATION

COLUSSI, NARELLA ANTONINA; TODARO, JUAN SANTIAGO; RODRÍGUEZ, JUAN PABLO; OLEA, GABRIELA BEATRIZ; FERRINI, LEANDRO ADRIAN; STOYANOFF, TANIA ROMINA; AGUIRRE, MARÍA VICTORIA

MEDICINA (BUENOS AIRES)

MEDICINA (BUENOS AIRES)

Lugar: Buenos Aires; Año: 2023 vol. 2023

0025-7680

Introduction: Chia and flax seeds are rich in alphalinolenicacid (ALA), which is bioconverted into theactive derivatives eicosapentaenoic (EPA) and docosahexaenoic(DHA) having multiple beneficial effects.However, there is limited knowledge about the antiinflammatoryeffects of chia and flax integral floursdiets rich in ALA.Objective: The study aimed to evaluate the antiinflammatoryeffect of dietary supplementation withintegral chia and flax flours in a murine model of LPSinducedsystemic inflammation.Methods: Balb/c mice were distributed into threegroups: diet A (control), diet B (supplemented with integralchia flour), and diet C (supplemented with integralflax flour). Nutritional, hematological, and biochemicaldeterminations were performed. ALA, EPA, and DHAwere assessed by GC-MS in the liver, brain, cardiacand skeletal muscles. NF-kB immunoassays were performedin kidney, liver, and peritoneal macrophages,respectively. The phagocytic capacity was determinedin peritoneal macrophages and the expression of thepro- and anti-inflammatory cytokines was assessed byRT-qPCR in the kidney, liver, and spleen.Results: Diets B and C exhibited optimal nutritionaladequacy and caused increased levels of ALA, EPA, andDHA in critical tissues compared to the control. Thephagocytic capacity of murine peritoneal macrophages(p< 0.01) and IL-10 transcription increased, whereas theexpression of NF-κB, IL-1β, IL-6, and TNF-α decreased inanimals fed both experimental diets.Conclusions: This work contributes to the currentknowledge of the anti-inflammatory effects of chia andflax integral flours rich in ALA and reinforces the healthadvantages of their consumption.