BECAS
NICOLA Maribel
artículos
Título:
Thermally self-assembled biodegradable poly(casein-g-N-isopropylacrylamide) unimers and their application in drug delivery for cancer therapy
Autor/es:
CUGGINO, JULIO C.; AMBROSIONI, FRANCO E.; PICCHIO, MATÍAS L.; NICOLA, MARIBEL; JIMÉNEZ KAIRUZ, ÁLVARO F.; GATTI, GERARDO; MINARI, ROQUE J.; CALDERÓN, MARCELO; ALVAREZ IGARZABAL, CECILIA I.; GUGLIOTTA, LUIS M.
Revista:
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2020 vol. 154 p. 446 - 455
ISSN:
0141-8130
Resumen:
In this work, we report the synthesis of graft copolymers based on casein and N-isopropylacrylamide, which canself-assemble into biodegradable micelles of approximately 80 nm at physiological conditions. The obtained copolymerswere degraded by trypsin, an enzyme that is overexpressed in several malignant tumors. Moreover,graft copolymerswere able to load doxorubicin (Dox) by ionic interactionwith the casein component. In vitro releaseexperiments showed that the in situ assembled micelles can maintain the cargo at plasma conditions butrelease Dox immediately after their exposition at pH 5.0 and trypsin. Cellular uptake and cytotoxicity assays revealedthe efficient delivery to the nucleus and antiproliferative efficacy of Dox in the breast cancer cell lineMDA231. Both delivery and therapeutic activity were enhanced in presence of trypsin. Overall, the prepared micelleshold a great potential for their utilization as dual responsive trypsin/pH drug delivery system
