BECAS
SALAS Nehuen
artículos
Título:
VPS32, a member of the ESCRT complex, modulates adherence to host cells in the parasite Trichomonas vaginalis by affecting biogenesis and cargo sorting of released extracellular vesicles
Autor/es:
SALAS, NEHU√ČN; COCERES, VERONICA M.; MELO, TUANNE DOS SANTOS; PEREIRA-NEVES, ANTONIO; MAGUIRE, VANINA G.; RODRIGUEZ, TANIA M.; SABATKE, BRUNA; RAMIREZ, MARCEL I.; SHA, JIHUI; WOHLSCHLEGEL, JAMES A.; DE MIGUEL, NATALIA
Revista:
CELL. MOLEC. LIFE SCIEN.
Editorial:
Springer Science and Business Media Deutschland GmbH
Referencias:
Año: 2022 vol. 79
ISSN:
1420-682X
Resumen:
Trichomonas vaginalis is a common sexually transmitted extracellular parasite that adheres to epithelial cells in the human urogenital tract. Extracellular vesicles (EVs) have been described as important players in the pathogenesis of this parasite as they deliver proteins and RNA into host cells and modulate parasite adherence. EVs are heterogeneous membrane vesicles released from virtually all cell types that collectively represent a new dimension of intercellular communication. The Endosomal Sorting Complex Required for Transport (ESCRT) machinery contributes to several key mechanisms in which it reshapes membranes. Based on this, some components of the ESCRT have been implicated in EVs biogenesis in other cells. Here, we demonstrated that VPS32, a member of ESCRTIII complex, contribute to the biogenesis and cargo sorting of extracellular vesicles in the parasite T. vaginalis. Moreover, we observe that parasites overexpressing VPS32 have a striking increase in adherence to host cells compared to control parasites; demonstrating a key role for this protein in mediating host: parasite interactions. These results provide valuable information on the molecular mechanisms involved in extracellular vesicles biogenesis, cargo-sorting, and parasite pathogenesis.