EXTRACELLULAR VESICLES SPREAD ADAPTIVE PRO-INFLAMMATORY RESPONSES TRIGGERED BY TRIIODOTHYRONINE (T3) ON MICE DENDRITIC CELLS (DCS)

NEGRETTI- BORGA DANA MARÍA; ALAMINO VANINA ALEJANDRA; SOLER MARÍA FLORENCIA; BRAVO-MIANA ROCÍO DEL CARMEN; BLANCO ANTONELLA; DONADIO ANA CAROLINA; MONTESINOS MARÍA DEL MAR; PELLIZAS CLAUDIA GABRIELA

Congreso; Reunión anual de sociedades de Biociencia. SAIC- SAI- SAFIS; 2020

SAIC- SAI- SAFIS

T3 is the biologically more active thyroid hormone. DCs are specializedantigen presenting cells. T3 activates mice DCs (T3-DCs)inducing Th1 and Th17 proinflammatory, and cytotoxic responses,restraining Tregs. On this basis, antitumor antigen-specific T3-DCsbased vaccination strategies were exploited successfully for melanomaand colon cancer in mice. Extracellular vesicles (EVs) arenano-sized membrane vesicles that play key roles in intercellularcommunication. We reported that EVs released by T3-DCs (T3-EVs)activated syngeneic DCs with a pro-inflammatory profile, contributingto paracrine DC communication in vitro. To go further, the aim ofthis study was to evaluate the adaptive modulatory role of T3-EVs.Bone marrow DCs obtained from C57BL/6 WT mice were stimulated(or not) with T3 (10nM) for 18h. DC-EV fractions were isolated bydifferential ultracentrifugation of culture supernatants (2,000g: 2K;10,000g: 10K; and 100,000g: 100K). Allogenic splenocytes wereobtained from BALB/c mice and stimulated with DC-EV fractionsof T3-stimulated and Control (C) cells for 6 days. Intracellular andsecreted cytokine production were analyzed by flow cytometryand ELISA. Statistical analysis: Sidak?s multiple comparisons test.P