HO-1 Genetic Variants and its Effects in Thyroid Cancer Biology

ALONSO, E.G.; MASCARÓ, M.; SCHWEITZER, K.; FERNÁNDEZ CHÁVEZ, L.; COLÓ, G.P.; ALONSO, E.N.; FERRONATO, M.J.; FERMENTO, M.E.; ROSEMBLIT, C.; CURINO, A.C.; FACCHINETTI, M.M.

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencia; 2023

In previous studies on human thyroid cancer (TC), we observed elevated levels of hemeoxygenase-1 (HO-1) protein in both cytoplasmic and nuclear compartments. Additionally, increased HO-1 mRNA expression correlated with tumor progression. Activation of HO-1 through hemin treatment in the TPC-1 cell line promoted cell viability, proliferation, migration, and cell cycle progression, while inhibition via ZnPP had opposite effects. This study aimed to investigate the impact of genetically overexpressed HO-1 variants (full-length - FL, enzymatically inactive - H25A, and nuclear truncated - t-HO1) on cancer-related processes. Using stable transfections of these variants into TPC-1 cells, we observed that FL and H25A forms were predominantly overexpressed in the cytoplasm, while t-HO-1 accumulated in the nucleus. Overexpression of FL and t-HO-1 significantly enhanced cell viability (p