Proteomic study of breast cancer cell line after hemeoxygenase-1 modulation by hemin treatment

SCHWEITZER, K.; FERNÁNDEZ CHÁVEZ, L.; ALONSO, E.G.; MASCARÓ, M.; ORESTI, G.M.; BORISOV, A.; FACCHINETTI, M.M.; CURINO, A.C.; FÄSSLER, R.; COLÓ, G.P.; GANDINI, N.A.

Mar del Plata

Congreso; Reunión anual de sociedades de biociencia; 2019

Hemoxygenase-1 (HO-1) is a microsomal enzyme that catalyzes the degradation of the heme group and it can be translocated to multiple subcellular compartments. Our laboratory, among others, has shown that HO-1 regulates several processes related to cancer progression such as: proliferation, invasion migration, metastasis and the epithelial-mesenchymal transition. The aim of this work is to investigate the role of HO-1 in the proteome modulation in a breast cancer cell line. Protein extracts of LM3 cell line treated with hemin, a pharmacological HO-1 inducer (80 UM, 24h), were obtained and studied by Western blot and Mass Spectrometry (MS). MS-data analysis showed 1033 from 7292 proteins were modulated after hemin treatment (ANOVA p