HEMEOXYGENASE-1 PLAYS A PROTUMORAL ROLE IN THYROID CANCER

ALONSO, E.G.; PICHEL, P.; MASCARÓ, M.; SCHWEITZER, K.; FERNÁNDEZ CHÁVEZ, L.; COLÓ, G.P.; RECIO, S.; ROSEMBLIT, C.; FACCHINETTI, M.M.; CURINO, A.C.

Congreso; Reunión Anual de Sociedades de Biociencia; 2021

Sociedad Argentina de Investigaciones Clínicas

We have previously demonstrated that hemeoxygenase-1 (HO-1) mRNA is overexpressed in papillary (PTC) and anaplastic (ATC) thyroid tumor compared to non-malignant areas to the tumor (NMT). We also demonstrated that HO-1 protein levels are up-regulated in human PTC samples, showing cytoplasmic localization, and such HO-1 tumor expression correlates with histological subtype. Now, we aim to study the role of HO-1 in thyroid cancer (TC) biology. To that end, we evaluated HO-1 expression by indirect immunofluorescence, cell viability by crystal violet method, cell cycle progression by propidium iodide staining and flow cytometry, and cell migration by wound healing assay. We found that pharmacological activation of HO-1 using 80μM hemin increased cell viability in TPC-1 (p