A FIRST CYTOGENETIC MAP OF YELLOW PASSION FRUIT (Passiflora edulis SIMS, PASSIFLORACEAE)

SADER MA, MUNHOZ, C. (CARLA MUNHOZ); PENHA, H. (HELEN PENHA), BERGES, H. (HELENE BERGÈS); VIEIRA, M. L. C. (MARIA LÚCIA CARNEIRO VIEIRA); PEDROSA-HARAND, A. (ANDREA PEDROSA-HARAND)

Montevideo

Congreso; Congresso Latinoamericano de Genética; 2016

Sociedad Argentina de Genética

Passiflora is the largest genus of the Passifloraceae family and includes about 500 species. The Neotropics are the center of diversity for the genus, and more than 130 Passiflora species are native to Brazil. The passion fruit (P. edulis, 2n = 18) is the major crop because of its commercial interest for juice production and fresh fruit consumption. Despite the recent advances in genetic and genomic studies, it has not been possible to identify each of the chromosomes of P. edulis. Chromosome-specific markers can be developed using fluorescent in situ hybridization (FISH) and genomic sequences inserted in bacterial artificial chromosomes (BACs) as probes. In this study, 27 gene-containing BACs of P. edulis, as well as ribosomal DNA (rDNA) and three retrotransposon sequences, were used as probes to construct a physical map of passion fruit by FISH. Together with the 5S rDNA site, 12 single-copy sequences were mapped, mainly in terminal chromosome regions, allowing the identification of eight chromosome pairs. Furthermore, a dispersed distribution of retrotransposons Ty1-copia, Ty3-gypsy and LINE was demonstrated. Our results have confirmed the importance of BAC-FISH for chromosome characterization and identification and provided a cytogenetic map with chromosome-specific markers for passion fruit.