Impact of Moderate Zinc Deficiency During Fetal Life and Postnatal Growth on Cardiac Nitric Oxyde System, Inflammatory and Oxidative Markers in Adult Male Rats

LORENA VANESA JURIOL; MARÍA NATALIA GOBETTO; FACUNDO MENDES GARRIDO ABREGÚ; GONZALO PINEDA; MARINA ERCILIA DASSO; JORGE EDUARDO TOBLLI; ANDREA CARRANZA; ROSANA ELESGARAY; ANALÍA LORENA TOMAT; CRISTINA TERESA ARRANZ

Boston

Congreso; Congress of Experimental Biology 2015; 2015

American Association of Anatomists (AAA), Amerinca Physiological Society (aps), American Sociesty for Biochemistry and Molecular Biology (ASBMB), ASIP, American Society of Nutrition (ASN) and ASPET

Moderate zinc deficiency during fetal and postnatal growth programs cardiovascular dysfunction and hypertension. Male zinc deficient rats showed reduced left ventricular (LV) wall thickness and ejection fraction. Aim: to evaluate nitric oxide (NO) system, pro-inflammatory cytokines and oxidative state in LV of adult male rats exposed to this deficiency. Wistar rats received during pregnancy up to weaning low (L:8 ppm) or control (C:30 ppm) zinc diet. After weaning, male offspring fed low (l) or control (c) zinc diet during 60 days (Cc, Ll, Lc). At day 81, we evaluated in LV: Interleukin-6 and Tumor Necrosis Factor-α (IL-6;TNF-α), basal NO synthase (NOS) and endothelial, neuronal and inducible (eNOS;nNOS;iNOS) isoforms activities; eNOS and Ser1177 eNOS phosphorylation protein expression (eNOS/β-actin; pSer1177eNOS/eNOS), mRNA expression (eNOS/GAPDH) and lipid peroxidation end products (TBARS). Means±SEM, n=6/group. One way ANOVA, Bonferroni post-test.TNF-α (immunohistochemistry;% of positive staining/area): Cc: 1.4±0.4; Ll: 20.4±2.0*; Lc: 1.7±0.3; IL-6 (immunohistochemistry; % of positive staining/area): Cc: 1.8±0.3; Ll: 21.1±1.7*; Lc: 2.8±0.8; Basal NOS Activity (pmol14C L-citrulline/g tissue.min): Cc: 204±6; Ll: 164±10*; Lc: 157±11*; Basal NOS+iNOS inhibitor (Aminoguanidine; pmol14C L-citrulline/g tissue.min): Cc: 216±7; Ll: 166±8*; Lc: 163±9*; Basal NOS+nNOS inhibitor (7-nitroindazole; pmol 14C L-citrulline/g tissue.min): Cc: 209±10; Ll: 166±8*; Lc: 164±9*; Basal NOS+Ca2+-calmodulin inhibitor (Calmidazolium; pmol 14C L-citrulline/g tissue.min): Cc: 47±2#; Ll: 52±2#; Lc: 49±2#;pSer1177eNOS/eNOS (Western Blot; optical density relative to Cc): Cc: 1.00±0.02; Ll: 0.74±0.03*; Lc: 1.02±0.16;TBARS (nmol/mg protein): Cc: 0.21±0.02; Ll: 0.78±0.08*; Lc 0.47±0.02*?.No differences were found in eNOS protein and mRNA expression.*p