Gender differences in cardiovascular alterations induced by Zinc restriction during fetal life and growth.

CRISTINA TERESA ARRANZ; LORENA VANESA JURIOL; FACUNDO MENDES GARRIDO ABREGÚ; MARÍA NATALIA GOBETTO; ROSANA ELESGARAY; M MAC LAUGHLIN; MARINA ERCILIA DASSO; DANIELA CARDELLI ALCALDE; JUDITH ZILBERMAN; MARÍA DE LOS ÁNGELES COSTA; ANALÍA LORENA TOMAT

Milán

Congreso; 23nd European Meeting on Hypertension; 2013

European Society of Hypertension

Male adult rats exposed to zinc restriction in the diet during fetal life showed hypertension, renal dysfunction and alteration in lipid metabolism in the adult life. Meanwhile, 6 and 21 days-old rats showed myocytes remodelling and reduced cardiac nitric oxide synthase activity. Objective: To evaluate cardiovascular alterations in growing and adult zinc deficient rats and to compare the effects in males (m) and females (f) rats. Design and method: Wistar rats were exposed from the beginning of pregnancy up to adulthood: low (L, 8 ppm) or control(C, 30 ppm) zinc diet. At day 81, we evaluated, in m and f offspring of each group of mothers (Cm, Lm, Cf and Lf), systolic blood pressure (SBP, mmHg) by indirect tail-cuff technique, thoracic aorta diameter (AD,μm, stained by heamatoxylin-eosin), heart weight/tibial lengh (HW,mg/mm) , and left ventricular (LV) function, assessed by echocardiography, studying end ?diastolic (EDV, ml) and systolic (ESV,ml) volume and ejection fraction (EF %). Results: Zinc deficient rats showed reduced HW in both gender (Cm: 30.9±1.3, Lm: 27.3 ± 1.1*; Cf: 25.1±0.5, Lf:19.9±0.7?). AD was reduced only in m zinc deficient rats (Cm: 1728±34; Lm:1468±41*; Cf:1551±42; Lf:1536±33, NS). Table: SBP: Cm: 122±2; Lm: 141±5*; Cf: 120±2; Lf: 122±5. HW/TL: Cm: 30,9±1,3; Lm: 27,3±1,1*; Cf: 25,1±0,5; Lf: 19,9±0,7?. EDV: Cm: 0,14±0,01; Lm: 0,24±0,02*; Cf: 0,17±0,02; Lf: 0,12±0,01. ESV: Cm: 0,02±0,01; Lm: 0,08±0,01*; Cf: 0,04±0,01; Lf: 0,03±0,01. EF%: Cm: 79±7; Lm: 66±4*; Cf: 78±1; Lf: 77±3. Values are means±SEM, n=6 for each group.Two ways ANOVA, Bonferroni post test *p