LIRAGLUTIDE REGULATES SERTOLI CELL ENERGETIC METABOLISM.

DASSO, M.E.; CENTOLA, C.L.; GALARDO, M.N.; MERONI, S.B.; RIERA, M.F.

Valdivia, Valdivia

Congreso; V Meeting Latin American Chapter Developmental Origins of Health and Disease (DOHaD) Society - XXXIV Meeting Chilean Society of Reproduction and Development; 2023

DOHaD Society

Background: Sertoli cells (SC) provide the nutritional support for developing germ cells. They metabolize glucose to lactate, which is delivered to germ cells to be used as preferred energy substrate. Besides, SC can use fatty acids (FA) as source of energy and can store them as triacylglycerols (TAG) within lipid droplets (LD). The LD content is important to maintain energetic reserve in SC. Obesity prevalence has risen dramatically and liraglutide, a glucagon-like peptide-1 (GLP-1) analogue, has emerged as a useful drug for treatment of obesity and type 2 diabetes mellitus. It has been observed that liraglutide regulates cell metabolism in different cell types. Considering that the regulation of FA storage may be relevant to seminiferous tubule physiology, the aim of this work was to assess if liraglutide regulates lactate production and LD storage in SC.Methods: Twenty-day-old rat SC cultures were incubated with liraglutide (10 or 100 nM) for various periods of time.Results: We showed that liraglutide does not modify glucose consumption, glucose transporter 1 expression or lactate production. However, liraglutide increases TAG levels and LD content (p