INVOLVEMENT OF GLUTAMINOLYSIS IN THE REGULATION OF SERTOLI CELL PROLIFERATION

CECILA CENTOLA; GUSTAVO RINDONE; AGOSTINA GORGA; MARINA ERCILIA DASSO; ELIANA PELLIZARI; MARIA DEL CARMEN CAMBEROS; FERNANDA RIERA; SILVINA MERONI; MARIA NOEL GALARDO

Mar del Plata, Provincia de Buenos Aires

Congreso; LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2021

Sociedad Argentina de Investigación Clínica (SAIC)

The final number of Sertoli cells (SC) reached during the proliferative periods determines sperm production capacity in adulthood. FSH is the major SC mitogen and mTORC1/p70S6K pathway is involved in FSH-stimulated SC proliferation. On the other hand, glutaminase (GLS), which converts glutamine into glutamate, plays a vital role in up-regulating cell metabolism for cell growth, and even, the glutamine catabolism might be required for the fully activation of mTORC1/p70S6K pathway. Previously, we have demonstrated that FSH increases the expression of GLS isoform 2 in proliferating SC, however, the role of GLS activity in the regulation of SC proliferation remains unknown. The aim of this work was to analyze whether SC depends on glutaminolysis to proliferate. SC obtained from 8-day old rats were maintained in the absence or presence of a pharmacological inhibitor of GLS -6-diazo-5-oxo-L-norleucine (DON) 500 μMunder basal conditions (B) or stimulated with FSH 100ng/ml. BrdU incorporation, c-Myc transcriptional activity by luciferase reporter assay after cell transfection and phosphorylated p70S6K (P-p70S6K) levels by western blot were evaluated. Results are expressed as mean±SD of three independent experiments (Different letters indicate statistically significant differences, P