ASSESSMENT OF THE MECHANISMS UNDERLYING ACTIVIN A-INDUCED SERTOLI CELL PROLIFERATION

GUSTAVO RINDONE; CECILA CENTOLA; AGOSTINA GORGA; MARINA ERCILIA DASSO; ELIANA PELLIZARI; MARIA DEL CARMEN CAMBEROS; MARIA NOEL GALARDO; SILVINA MERONI; FERNANDA RIERA

Mar del Plata, Provincia de Buenos Aires

Congreso; LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2021

Sociedad Argentina de Investigación Clínica (SAIC)

Immature Sertoli cells (SC) proliferate to reach a steady population size that will ultimately correlate with sperm output in adulthood. Activin A (ActA), a member of the TGF-β superfamily, acts as a paracrine factor on fetal and postnatal proliferation of SC. However, besides an increase in Cyclin D2 expression, little is known about the mechanisms that explain ActA-induced proliferation of SC. The aim of this study was to assess the signaling pathways and gene expression changes involved in ActA-induced proliferation of SC. SC were isolated from 8-dayold Sprague-Dawley rats and incubated under basal conditions (B) or stimulated with ActA (50 ng/mL) during different periods of time. Results are expressed as X±SD, n=3 (*p