Potential of MALDI-TOF as a diagnostic tool in sepsis

TODERO, MARÍA FLORENCIA; BÁRBARA REARTE; YOKOBORI, NOEMÍ; MONICA PRIETO

Mar del Plata

Congreso; SAI; 2018

Sepsis has a bi-phasic naturewith a hyper-inflammatoryphase followed by an anti-inflammatory response. Globally, it is one of the main causes of death in ICUs. However, clinically, is difficult to definea therapeutic approach due to the lack of toolsto determine the phase that the patient is going through. Our aim was to investigate the usefulnessof plasma ?fingerprints? using MADI-TOF MS technology as rapid instrument for determination of pro-inflammatory or anti-inflammatory states in sepsis. For this, LPS-challenged murine modelsemulating the different phases of sepsis (proinflammatory (PRT) and anti-inflammatory (ANT) phases) versus untreated controls (Ctrl) wereevaluated. To better characterize the different phases, in parallel, cytokines profiles, hematologic, metabolic and immunologic parameters were determined in plasma. Peripheral blood leukocytescount was decreased in PRT whereas in the ANT phase was increased mainly associated with an increase in neutrophils(Leukocytes(106/ml): Ctrl=6.6±0.5; PRT=1.9±0.2*; ANT=11.2 ±0.7&; *&p