INVESTIGADORES
VARANI AndrÉs Pablo
congresos y reuniones científicas
Título:
Histopathological, inmunohistochemical and biological characterization of the CFM-27 tumoral line
Autor/es:
LOMBARDO D; FIORITO C; QUIROGA S; DE SANDE C; VARANI A.; BUSTOS R; FORASTIER N.
Lugar:
Tandil, Buenos Aires
Reunión:
Congreso; X Congreso Argentino de Ciencias Morfológicas.; 2006
Institución organizadora:
Facultad de Ciencias Veterinarias. Universidad Nacional del Centro de la Provincia de Buenos Aires
Resumen:
Tumors obtained by induction in Wistar rats with n«nitroso n«methyl
urea (NMU), demonstrated an histopathological pattern corresponding
to an alveolar mammary adenocarcinoma. Those originated in
mice only treated with medroxiprogesterone (MPA) were ductal
mammary carcinomas. Inductions with NMU in mice Balb/c did
not reproduce the tumorigenic effect. The induction and later promotion
with MPA in mice Balb/c generated an alveolar mammary
adenocarcinoma. After several syngeneic passages of tumoral fragments
obtained by induction and promotion a tumoral line with
different histopathologic character was stablished. Mice of 5 weeks
were induced with three doses of NMU (50 mg/kg) and three doses
of MPA (40 mg). Two months after induction tumors were developed
and detectables by palpation. These were transplanted in syngeneic
form to female mice without inmunosuppression. They were
carried out 42 successive passages in Vivo and samples were frozen
at -120¼C to repeat passages starting from the same one. The
transplants were in the inguinal pleat. With a latency of 7 days they
were developed tumors locally, differing from the original pattern
to a sarcoma of small cells. With the histological routine Tricrhomics
were carried out and the cellular pattern was determined by
inmunohistochemistry, using membrane markers like Cadherin E,
N, Cytoqueratin P8 and Pan cytoqueratine. The expression of receptor
of hormon-dependence was evaluated with Progesterone
monoclonals anti receptor of and anti protein P29. Up to now the
CFM-27 line maintains its histopathologic pattern without changes
in successive passages, being able to be a tumoral model repetible
for the study of different biological aspects of neoplasia.