Proteome variation of the rat liver after static cold storage assayed in an ex vivo model

CAMILA KNECHT; CECILIA, L. BALABAN; JOAQUIN V. RODRIGUEZ; EDUARDO A. CECCARELLI; EDGARDO E. GUIBERT; GERMÁN L. ROSANO

CRYOBIOLOGY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 2018 vol. 85 p. 47 - 55

0011-2240

Cold storage is a common procedure for liver preservation in a transplant setting. However, during coldischemia, the liver suffers molecular alterations that can affect its performance. Also, deleterious mechanisms setforth in the storage phase are exacerbated during reperfusion. This study aimed to identify liver proteins associatedwith injury during cold storage and/or normothermic reperfusion using the isolated perfused rat livermodel. Livers from male rats were subjected to either (1) cold storage for 24 h, (2) ex vivo normothermic reperfusionfor 90 min or (3) cold storage for 24 h followed by ex vivo normothermic reperfusion for 90 min. Then,the livers were homogenized and proteins were extracted. Protein expression between each experimental groupand the control (freshly resected livers) was compared by two-dimensional (2D) gel electrophoresis. Proteinidentification was carried out by matrix‐assisted laser desorption/ionization time‐of‐flight spectrometry(MALDI‐TOF/TOF) using MASCOT as the search engine. 23 proteins were detected with significantly alteredlevels of expression among the different treatments, including molecular chaperones, antioxidant enzymes, andproteins involved in energy metabolism. Some of them have been postulated as biomarkers for liver damagewhile others had been identified in other organs subjected to ischemia and reperfusion injury. The whole data setwill be a useful resource for studying deleterious molecular mechanisms that result in diminished liver functionduring storage and subsequent reperfusion.