Artificial Insemination Out Of Breeding Season In Water Buffaloes In Argentina

JOSÉ KONRAD; RAMON CAMELINO; ROBERTO YUPOPNI; NATALIA VALLEJOS; ALEXIS BANDEO; JESUS BERDUGO; GUSTAVO CRUDELI; PABLO MALDONADO VARGAS

Estambul

Congreso; 12 º Congreso Mundial de Búfalo; 2019

Federación Internacional de Búfalo

Seasonality in the reproduction of water buffalo is one of the main problems of breeders to have a constant production. Some authors have beensuggested that the use of out of breeding season ovulation induction programs, might help to overcome this problem. The objective of this work was to evaluate the effect over pregnancy rates the application of an ovulation synchronization protocol designed for out of breeding seasons. Thiswork was performed in September of 2018, during out of breeding season in a farm located in Corrientes, Argentina. 26 adult normal females ofsimilar weight and parity were synchronized, using progesterone, eCG, prostaglandin and estradiol cipionate protocol. Ten days before the startingthe synchronization protocol half of the animals were treated with melatonin implants at a dose of 18mg/50 kg of live weigh. At the beginning ofthe protocol blood sample to determine antimüllerian hormone (AMH) was taken. Inseminated by the same technician with frozen semen ofMurrah bulls of proved fertility. Pregnancy rates were compared with those obtained during breeding season artificial insemination protocols inthe same farm. Data were analyzed using comparison of proportions, using Tukey test and statistical difference were considered p>0.05. 9 of the26 females became pregnant (34.61%) compared to (52.5%) during breeding season, with no statistical difference (p=0.2076). No difference inAMH levels were found between pregnant and non-pregnant females, 245.5 vs 238.7 pg/ml (p=0.1496). No effect of male in pregnancy rates were