Photophysics and photochemistry of mercurochrome in reverse micelles

MARCELA S. ALTAMIRANO; MICAELA E. GRASSANO; SONIA G. BERTOLOTTI; CARLOS M. PREVITALI

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY

ELSEVIER SCIENCE SA

Lugar: Amsterdam; Año: 2016 vol. 329 p. 149 - 154

1010-6030

The photophysics of the xanthene dye 20,70-Dibromo-50-(hydroxymercurio)fluorescein disodium salt (merbromine, mercurochrome) (MCr) was studied in reverse micelles (RMs) of sodium 1,4-bis (2-ethylhexyl) sulfosuccinate (AOT) and benzylhexadecyldimethylammonium chloride (BHDC). In the reverse micellar media both the absorption and emission spectra of the dye present a red shift with respect to water. It is concluded that MCr in both reverse micellar systems is localized in the interface. InBHDC due to the positive charge at the interface and the negative charge of the dye, it remains anchored to the interface irrespective of the water content of the RMs. On the other hand in the case of the negative interface of AOT the photophysical properties tend to those in homogeneous water solution as the water content increases. For small size AOT RMs the dye resides in the interface co-micellizing with the surfactant, although more exposed to water than in the case of BHDC. Special interest was assigned to the effect of confinement on the triplet state properties. The triplet state decay kinetics and absorption spectra were determined by laser flash photolysis. The triplet lives longer in RMs than in homogeneoussolvents. The compartmentalization of the dye precludes the auto-quenching which shorts the lifetime in homogeneous solvents. Furthermore, the quenching by electron donors and acceptors was investigated. Two aliphatic amines were used as electron donors. The hydrophilic triethanolamine (TEOA) is localized in the water pool, and the triplet quenching is more efficient than in homogeneous solvents. The apparent quenching rate constant for TEOA in AOT is one order of magnitude higher than BHDC. On the other hand the quenching by dipropylamine (DPA) which is located in the bulk organic solvent is much less efficient. The quenching by TEOA in RMs can be understood in terms of an exchange mechanism between micelles. As electron acceptor 1,4-benzoquinone (BQ) was employed, and the value in BHDC is two orders of magnitude higher than in AOT. These divergences most likely arise from different partitions of the quenchers in the reverse micellar systems.