LIPOPOLYSACCHARIDE OF THE HYPEREPIDEMIC CLONE CARBAPENEM RESISTANT Klebsiella pneumoniae IMPAIRS THE RESPIRATORY BURST INDUCED BY FMLP IN HUMAN NEUTROPHILS

CASTILLO, LUIS A.; RODRIGUEZ-RODRIGUES, NAHUEL; MARTIRE-GRECO, DAIANA; BIRNBERG WEISS, FEDERICO; SCHIERLOH, PABLO; LANDONI, VERONICA I.; FERNANDEZ, GABRIELA C.

Congreso; LXV Reunión Anual de la Sociedad Argentina de Inmunología 2017 - Reunión Conjunta de Sociedades de Biociencias; 2017

Klebsiella pneumoniae (Kp) is a widespread, opportunistic pathogen associated with high mortality rates in immunocompromised patients, attributed to the production of Kp-Carbapanemase (KPC), an enzyme that mediates resistance to carbapenems. Previously, we have demonstrated that Kp-KPC sequence type 258, a multi-resistant hyperepidemic clone, induced poorly bactericidal responses in PMN and Kp bacterial extracts (KpBE) were able to subvert reactive oxygen species (ROS) production induced by fMLP. In this work, our aim was to study the mechanisms and bacterial components involved in this ROS inhibition. Using isolated human PMN we found that KpBE also reduced ROS production induced by another seven trans-membrane agonist, Leukotiene B4 (LB4), measured with dihydrorhodamine 123 (DHR) by FACS (%DHR+ PMN: LB4=41,4±4; KpBE+LB4=23,9±5, p