INDUCED PLURIPOTENT STEM CELL-DERIVED MESENCHYMAL STEM CELL DECREASE ENDOTHELIAL DAMAGE CAUSED BY SHIGA TOXIN TYPE 2

MARTIRE-GRECO, DAIANA; LUZZANI, CARLOS; CASTILLO, LUIS A.; LA GRECA, ALEJANDRO; FURMENTO, VERONICA; BIRNBERG WEISS, FEDERICO; MIRIUKA, SANTIAGO GABRIEL; LANDONI, VERONICA I.; FERNANDEZ, GABRIELA C.

Mar del Plata

Congreso; LXVI Reunión Anual de la Sociedad Argentina de Inmunología 2018; 2018

SAI SAIC SAFIS

Shiga toxin 2 (Stx2) is key in microangiopathic events that occur inHemolytic Uremic Syndrome (HUS). Exposure to lipopolysaccharide(LPS) and Stx2, cause endothelial damage in the renal glomerulusinducing one of the most relevant issues that come to kidney fail-ure in this disease. Mesenchymal stem cells (MSC) are multipotentcells that possess known tissue regenerative properties. InducedPluripotent Stem Cell-Derived Mesenchymal Stem Cell (iPSC-MSC)has similar characteristics to Mesenchymal Stem Cell (MSC). Ourobjective was to study if iPSC-MSC could reduce the endothelialdamage/dysfunction caused by LPS and Stx2 exposure. For thispurpose, we used an in vitro model of endothelial damage usingHUVEC cells incubated with LPS and/or Stx for 24 h. We foundthat iPSC-MSC exposed to LPS decreased their migratory capacitymeasured as the migrated area after a wound in the cell monolayercompared to Control cells, but the combination of LPS+Stx reversedthis effect (area cm2(x102), Control: 5,1±0,4; LPS: 3,6±0,2*; Stx:3,8±0,3*; LPS+Stx: 6,5±0,4*,*vs. Control, p