INDUCED PLURIPOTENT STEM CELL-DERIVED MESENCHYMAL STEM CELL RESPONSE TO BACTERIAL LIPOPOLYSACCARIDE AND SHIGA TOXIN

MARTIRE-GRECO D; LA GRECA, ALEJANDRO; CASTILLO LA; BIANI, C; LOMBARDI, A; BIRNBERG WEISS F; NORRIS, A; RODRIGUEZ-RODRIGUES, NAHUEL; PITTALUGA JR; FURMENTO, VERONICA; LANDONI VI; MIRIUKA, SANTIAGO GABRIEL; LUZZANI, CARLOS; FERNÁNDEZ GC

Congreso; LXIX Reunión Anual de la Sociedad Argentina de Inmunología 2019; 2021

Mesenchymal Stem Cells can be activated and respond to different bacterial toxins. Lipopolysaccharides (LPS) and Shiga Toxin (Stx) are the two main bacterial toxins present in Hemolytic Uremic Syndrome (HUS) that cause endothelial damage. In this work we aimed to study the response of induced Pluripotent Stem Cells derived Mesenchymal Stem Cells (iPSC-MSC) to LPS and/or Stx and its effect on the restoration of injured endothelial cells. For this purpose, we stimulate iPSC-MSC with LPS and/or Stx for 24 h. using Polymyxin B in the Stx treatments in order to avoid LPS contamination. The results obtained showed that LPS induced a pro-inflammatory profile on iPSC-MSC with an increment of IL-8 and TNF-a, but not Stx when we measure with ELISA kit, (pg/ml IL-8 Control: 1988±299; LPS: 20876±1233*#, Stx: 1801±137; LPS+Stx:17935±213* and pg/ml of TNF-a Control:880±32; LPS:3291±116*# Stx:627± 8; LPS+Stx:2092 ±59*, *vs. Control, # vs Stx p