Exploring synteny relationships of Hordeum chilense genome for tritordeum breeding

ÁVILA, C. M.; MATTERA, M. G.; RODRÍGUEZ-SUÁREZ, C. ; PALOMINO, C. ; RAMÍREZ, M. C.; KILIAN, A.; HORNERO-MÉNDEZ, D.; ATIENZA, S. G.

Zaragoza

Simposio; I Simposio Español de Fisiología y Mejora de Cereales; 2018

Sociedad Española de Fisiología Vegetal, Sociedad Española de Genética, Asociación Española de Técnicos Cerealistas y Fundación Española de Cereales

Hexaploid tritordeum (×Tritordeum martini A. Pujadas (Pujadas 2016) has become a new crop due to its functional characteristics. The most important one is the golden colour of its flour which is caused by the presence of carotenoids (Atienza et al. 2007a). This new amphiploid was obtained after chromosome doubling of the hybrid between Hordeum chilense (Figure 1) and durum wheat (Martín & Sánchez-Monge 1982). H. chilense genes are responsible for the high carotenoid content (Atienza et al. 2007b; Rodríguez- Suárez et al. 2014) and the distinctive esterification profile of carotenoids (Mellado-Ortega and Hornero- Méndez 2015). The conservation of the carotenoid pathway has allowed the identification of the main genes responsible for the high carotenoid content in tritordeum (Atienza et al. 2007b; Mattera et al. 2015, Rodríguez-Suárez et al. 2014). However, this approach is not possible for processes where the pathway is not fully understood. Alternative strategies such as synteny-based candidate gene identification could be very useful in tritordeum breeding and related projects using barley genome as reference. In this work we aimed to investigate synteny relationships between H. chilense and barley through the development using DArTSeq markers. A good degree of collinearity was observed between both species with the exception of the pair 7H-7Hch were significant reorganization was observed.