CHARACTERIZING THE ROLE OF ALPHA-SYNUCLEIN IN MELANOMA

MALIZIA, FLORENCIA; ANSELMINO, LUCIANO; ZANOTTI, LUCÍA; MENACHO MÁRQUEZ, MAURICIO

Buenos Aires

Congreso; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2020

Sociedad Argentina de Investigación Clínica

The amyloid protein alpha synuclein (αS) is the main component of Lewy bodies, the neuropathological hallmark of Parkinson's disease (PD). The mechanisms underlying αS aggregation, neurotoxicity and cell-to-cell transmission were explored in the context of PD. Recent evidence suggests that αS may also play a putative role in melanoma, the most dangerous form of skin cancer. Current studies suggested that αS could be protective for advanced melanoma but its role in this type of cancer was not deeply explored yet.Previously, we demonstrated by in vitro studies that melanoma cells are able to uptake different aggregation species of αS. These species were not toxic for melanoma cells. Instead, they promoted proliferation, cytoskeleton rearrangement and migration. Here, we evaluated the ability of human SK-MEL 28 and mouse B16-F10 melanoma cells to form clones in the presence of αS fibers by standard colony forming assays. Cells were incubated (or not) with αS fibers for 24 hours and 300 cells were plated to form colonies. For both types of cells, incubation with αS promoted a higher number of clones (P