ALLOPREGNANOLONE INDUCES PROLIFERATION AND MIGRATION OF HUMAN OVARIAN CELL LINE IGROV-1 AND SKOV-3

SANHUEZA MA; PELEGRINA LT; CÉCERES ARR; RODRIGUEZ CE; LACONI MR

Mendoza

Congreso; Sociedad Biología de Cuyo; 2016

Ovarian carcinoma is one of the most common causes of cancer death from gynecologic tumors in the world. Allopregnanolone(ALLO) is an active metabolite of progesterone (P4) involved in physiological and reproductive parameters of female rat.Previously we showed that ALLO affects on the hypothalamic-pituitary-gonadal axis in response to stress inducing mooddisturbances and generating morphophysiological alterations in ovarian, being able to inhibit apoptosis and promote angiogenesisof corpora lutea. Furthermore, ALLO is able to inhibit ovulation and the formation of cystic structures. Epidemiological studiesas well as in vitro assays have shown that P4 has anti-tumor effects. However, the molecular mechanism of the anticancer effectof progestogen had not yet fully understood. In addition, the effect of ALLO in ovarian cancer is unknown. Our hypothesis is thatALLO affects tumor progression. In this work, we analyzed the proliferation and migration of human ovarian cancer cellsIGROV-1 and SKOV-3. To this end, both cell lines were exposed to different concentrations of ALLO (10-11-10-7 M) for 72 h andproliferation and migration were analyzed by MTS assay and wound assay respectively. We found that ALLO increasedproliferation in a concentration dependent manner, reaching a maximum effect of 80% (p