Recombinant antigens of Leishmania for the immunodiagnosis and immunoprophylaxis of american tegumentary leishmaniasis

MARCO JORGE DIEGO; BARROSO PAOLA ANDREA; ACUÑA LEONARDO; SANCHEZ-VALDEZ FERNANDO; FERNANDEZ ULLIVARRI MIGUEL; HOYOS CARLOS LORENZO; BRACAMONTE MARIA ESTEFANIA; AGUSTIN MOYA ALVAREZ; BELLOMIO AUGUSTO

Parana, Entre Rios

Congreso; 54th Annual Meeting Agentine Society for Biochemistry and Molecular Biology LIV Reunion Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2018

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

The leishmaniases are neglected parasitic diseases spread in 88 countries around the world, including Argentina, which control requires thedevelopment of new tools in diagnosis, vaccines and treatments. We applied the recently achieved Leishmania parasite genome/proteomeknowledge and recombinant DNA techniques for the selection and synthesis of polypeptides for designing of high performance diagnostics orimmunoprophylactics methods. Thus, through a seroproteomic analysis of the L. (V.) braziliensis amastigote extracts, three proteins were selected as candidates for immunodiagnosis. The criterion of selection applied was based on their differential reactivity againstsera from patientswith leishmaniasis and non-reactivity against sera from patients with Chagas disease, in two dimensional Western blots. In addition, fivecandidates for vaccines or immunotherapy were selected based on their differential induction of IgG2 or IgG3 antibodies detected by using serafrom patients with tegumentaryleishmaniasis. These subtypes of human immunoglobulin are related with a Th1 or Th2 type responses,determining the disease cure o progression respectively. After having expressed in Escherichia coli, these candidates have been analyzed forimmunodiagnostic purposes by imunobloting or ELISAs, or for vaccines development in murine models of tegumentaryleishmaniasis. This workdescribes the last results obtained, showing their applicability in the mentioned research fields.