Peptides from Amaranth controlled the NFkB pathway activation on epithelial cells and suppressed intestinal inflammation

SMALDINI, PAOLA; QUEREDA, MICAELA; RIZZO, GASTÓN; ORSINI DELGADO, MARÍA LUCÍA

Mar del Plata

Congreso; Reunión SAIC SAI SAFIS 2018; 2018

Biological, nutritional and health benefits of amaranth have been highlighted in the last years. Proteins of amaranth exert anti-hypertensive, anti-oxidant, anti-thrombotic and anti-proliferative effects. The aim of this study was to analyze the anti-inflammatory effect of peptides from amaranth on NF-kB-intracellular pathway activation in intestinal epithelial cells, and in experimental intestinal inflammation, such as colitis and food allergy.Colon cell lines (Caco-2 and Caco-luc) were cultured with flagellin and amaranth peptides. CCL20-expression was evaluated by qPCR and NF-κB modulation was evaluation by light emission and qPCR, along with p65-nuclear traslocation. In vivo studies included the oral administration of a formulation containing the peptide during the allergic sensitization or the colitis induction phase in Balb/c mice. Treatment efficacy was in vivo and in vitro evaluated.We found several peptides with anti-inflammatory capacity and we selected that with the highest ability to suppress cell activation (decrease in CCL20 and light emission p< 0.05). In vivo studies showed, an amelioration of the clinical score (p