Changes in neurosteroidogenesis in demyelination and remyelination

MARÍA LUZ LEICAJ; ANALÍA LIMA; LAURA PASQUINI; JUANA MARÍA PASQUINI; ALEJANDRO FEDERICO DE NICOLA; LAURA INÉS GARAY

Ciudad Autónoma de Buenos Aires

Congreso; The role of glial cells in health and disease of the nervous system: clinical and basic science walking together; 2017

Studies of Multiple Sclerosis and murine experimental autoimmune encephalomyelitis (EAE) have shown altered neurosteroidogenesis. We previously demonstrated that Progesterone pre-treatment decreases demyelination, neuroinflammation, improves clinical grade and re-establishes steroidogenic enzymes levels in the spinal cord of EAE mice (Garay 2007,2012,2017). Here, we asses hippocampal steroidogenic pathway in a cuprizone model of demyelination. We also analysed demyelination and neuroinflammation parameters in the hippocampus of control (CTRL), demyelinated (CPZ D), and remyelinated (CPZ R) mice. We found in CPZ D mice hippocampus decreased mRNA expression of the steroidogenic acute regulatory protein (STAR), P450scc (cholesterol side-chain cleavage), 5 a-reductase (CPZ D vs. CTRL;p