Hyperthyroidism enhances fetal and placental growth and placental immune cell infiltration

SÁNCHEZ MARÍA BELÉN; MORENO SOSA MARÍA TAMARA; NEIRA FLAVIA JUDITH; PIETROBON ELISA OLIVIA; SOAJE MARTA; VALDEZ SUSANA RUTH; JAHN GRACIELA ALMA; MACKERN OBERTI JUAN PABLO

Mar del Plata

Otro; Reunión conjunta de Sociedades de Biociencia; 2019

Thyroid dysfunctions cause reproductive disorders as fetal deaths, preterm birth and preeclampsia. Whether thyroid hormones (THs) exert any function in placentalimmune cells is unknown. Therefore, the aim of our work was to assess the influence of hyperthyroidism on placental immune cells as well as the impact on reproduction in pregnant rats. To this end, 10-12 weeks old Wistar rats were injected with a daily dose of T4 (0.1 or 0.25 mg/kg s.c) to induce hyperthyroidism (hyper) or vehicle in control animals. Rats were mated 8 daysafter starting T4 treatment and euthanized on day 19 (G19) and 20 (G20) of gestation. Placenta samples were minced to reach single cell suspension. Then, resident placental immune cells (CD45+) were analyzed by flow cytometry and mRNA content of hormone receptors by qPCR. Also, placental and fetus weightsand fetus number were measured. Hyper mothers delivered more fetuses compared to controls (p