Hypophysitis in non obese diabetic mice as a new tissue specific autoimmune response

MORENO SOSA MARÍA TAMARA; SOAJE MARTA; SÁNCHEZ MARÍA BELÉN; VALDEZ SUSANA; YÚDICA SEDANO FLORENCIA; NEIRA FLAVIA; PENNACCHIO GISELA; PIETROBON ELISA; JAHN GRACIELA ALMA; MACKERN OBERTI JUAN PABLO

Buenos Aires

Otro; Reunión Conjunta de Sociedades de Biociencias; 2017

Type 1 diabetes mellitus is a chronic organ-specific autoimmunedisease that results from the destruction of beta (β) cells in the pan-creatic islets and shows a pronounced leukocyte infiltration in thegland. Non-obese diabetic (NOD) mice develop autoimmune dia-betes approximately at 6 months of age in females, providing anexperimental model for human Type 1 Diabetes. They also displayadditional autoreactive immune responses against other glands in-cluding the prostate and thyroid. Furthermore, female NOD miceexhibit a lower fertility index compared to wild type mice, that de-velops approximately at the same time as diabetes. To determinewhether the low fertility of NOD mice is caused by an autoimmuneresponse against the hypophysis, we assessed the presence of leu-kocyte infiltration in the gland. To this end, the presence of CD45+(panleukocyte) cells in the hypophysis of 3 and 6 months old femaleBALB/c (control group) and NOD mice was analyzed by flow cy-tometry. An increase in leukocyte infiltration in the hypophysis from6 months old NOD mice was observed compared to the controls(0.05±0.04 % BALB/c mice vs 3.2±1.0% NOD mice; p