Expression and modulation of prolactin receptor in immune cells: Induction of mRNA and proteins by dexamethasone

MORENO SOSA MARÍA TAMARA; SÁNCHEZ MARÍA BELÉN; NEIRA FLAVIA JUDITH; SOAJE MARTA; PIETROBON ELISA OLIVIA; VALDEZ SUSANA RUTH; RIVERO VIRGINIA; JAHN GRACIELA ALMA; MACKERN OBERTI JUAN PABLO

Congreso; Reunion Anual SAIC; 2020

It is known that there is a relationship between hyperprolactinemiaand autoimmunity. However, the role of prolactin receptor (PRL-R)in autoimmune development is still controversial. The aim of thiswork is to analyze the expression of PRL-R in murine immune cells,and determine whether PRL-R are modulated during activationwith Concanavalin A (conA) and suppression with Dexamethasone(dexa) in healthy mice and three mice models for Lupus. For thisend, splenocytes from C57BL/6 (C57), and lupic FcgRIIb KO (RIIb),NZM, and MRL/MpJ-Faslpr (FAS) mice were stimulated with 1 μg/mlof conA or 1 μg/ml dexa and kept in culture for 24 hours. RNA fromsplenocytes was isolated using Trizol and retrotranscribed to cDNAto detect PRL-RL by qPCR. Protein determination was carried outby flow cytometry using Anti-CD4, Anti-CD19, and Anti-PRL-R. Wefound that splenocytes from RIIb mice have higher mRNA of PRL-RLexpression than other strains. In CD4+ T cells from C57 mice PRL-Rincreased by suppression with dexa and did no changes when stim-ulated with conA. In contrast, in CD4+ T cells from FAS mice, PRL-Rincreased after conA and decreased with dexa, while the CD4+ Tcells from RIIb and NZM mice displayed no changes. In CD19+B cells from C57 and FAS mice, we observed an increase of totalPRL-R when we stimulated with dexa and no changes with conA,while in CD19+ from RIIb and NZM mice, did not display changes.These results show a differential behavior of PRL-R expression inC57 mice compared with the lupic mice after stimulation with conAand dexa. The fact that C57 displayed low levels of PRL-R duringactivation but high in suppression gives us the notion that PRL-Rmay contribute to maintaining a cellular immune balance by limitingPRL trophic effects. By contrast, the high levels of PRL-R during ac-tivation in FAS mice suggest an exacerbated trophic effect of PRL.Our data show that differential PRL-R expression in immune cellsmay contribute to regulate the immune response.