LEUKOCYTES INFILTRATION CHAPERONES HYPERTHYROIDISM-INDUCED INCREASE OF FETAL GROWTH, PLACENTAL CHANGES, AND IMPAIRED OFFSPRING DEVELOPMENT

SÁNCHEZ MARÍA BELÉN; NEIRA FLAVIA JUDITH; MORENO SOSA MARÍA TAMARA; MICHEL MARÍA CECILIA; PIETROBON ELISA OLIVIA; SOAJE MARTA; VALDEZ SUSANA RUTH; JAHN GRACIELA ALMA; MACKERN OBERTI JUAN PABLO

Congreso; Reunión Anual de SOCIEDADES DE BIOCIENCIAS; 2021

Hyperthyroidism (HP) induces reproductive disorders like preterm birth and lactation failures and could influence immune cells homeostasis. Therefore, our work aimed to evaluate the role of thyroid hormones in leukocytes in milk and placenta. To this end, 12 weeks old Wistar rats were injected with T4 (hyper) and euthanized on day 19, 20 of gestation (G19 and G20), and day 2 of lactation (L2). Placenta and milk immune cells (CD45+, CD3+, CD11b/c+) were analyzed by flow cytometry, and mRNA hormone receptors and cytokines by qPCR. Histological analysis of the mammary gland was performed. We observed that fetuses of hyper group (hyper) weighed more in G19 and G20 (p