BECAS
SCARAFIA Maria Agustina
congresos y reuniones científicas
Título:
Cardiotoxicity studies in microfluidic devices using cardiomyocytes differentiated from human induced pluripotent stem cells
Autor/es:
ANAHÍ SANLUIS-VERDE; MARIA AGUSTINA SCARAFIA; IGOR DE SA CARNEIRO; ALEJANDRO DAMIÁN LA GRECA; GUSTAVO ROSERO; LUCIA NATALIA MORO; MAXIMILIANO S PEREZ; BETIANA LERNER; SANTIAGO GABRIEL MIRIUKA
Lugar:
Cordoba
Reunión:
Congreso; II Brazil - Argentine Microfluidics Congress; 2019
Institución organizadora:
Universidad Nacional de Cordoba
Resumen:
Microfluidic devices may have several advantages over traditional cell cultures,including replication of in vitro microenvironment and less needs of reagents.The objective of this work was to evaluate cardiotoxicity in our cardiac model in vitro using a microfluidic device with a dilution module.To test our experimental setting, cardiomyocytes (CMs: day 21) obtained bydifferentiation of human induced pluripotent stem cells (hiPSCs: day 0) were seeded in a culture chamber of microfluidic device to obtain an appropriate biophysical tissue architecture. The CMs were incubated with different concentrations of doxorubicin (DOX) created by the dilution module of microfluidic device starting from an initial concentration of 0.5 μM for 24 h with continuous flow (0.1 μl/min) to simulate the blood flow. We evaluated the cardiotoxicity by fluorescence microscopy after using live/dead assay.Our results showed that CMs obtained from differentiation protocol beatspontaneously at physiological rhythms (70-80 bpm). These CMs expressed cardiacmarker cardiotroponin (cTnT) detected by immunostaining and real-time qPCR andquantified by flow cytometry obtaining 80-90% of positive cells.By exposing the CMs to six different DOX gradient concentrations generated by the dilution module, we saw a direct relation between increase DOX concentration and CMs cell death.In conclusion, our ?lab on a chip? technology with hiPSC-CMs is a promising tool that could significantly improve the ability to test drug efficacy and toxicity in vitro, due to the use of human cells, easy use, reducing cost and duration with respect to conventional methods.
