Genome-wide identification of differentially expressed lncRNAs in cardiac differentiation from human induced pluripotent stem cells

MARIA AGUSTINA SCARAFIA; ALAN MIQUEAS MÖBBS; NATALIA LUCÍA SANTÍN VELAZQUE; GABRIEL NEIMAN; CAROLINA COLLI; ARIEL WAISMAN; LUCIA NATALIA MORO; GUSTAVO EMILIO SEVLEVER; CARLOS DANIEL LUZZANI; ALEJANDRO DAMIÁN LA GRECA; SANTIAGO GABRIEL MIRIUKA

Buenos Aires

Congreso; Latin American Society for Developmental Biology Meeting 2019; 2019

Latin American Society for Developmental Biology (LASDB)

Differentiation of pluripotent stem cells (PSC) into cardiomyocytes is a resourceful strategy to gain insight into gene regulatory networks that govern cardiac development and commitment. Recently, long non-coding RNAs (lncRNAs) have been shown to take part as important players of these networks by fine tuning epigenetic, transcriptional and post-transcriptional gene expression, although there is still much to unravel about their precise molecular mechanisms.The aim of this study was to thoroughly characterize lncRNAs genes that are differentially expressed in cardiac differentiation from human induced PSC.We performed RNAseq on three cellular populations: PSC, early mesoderm progenitor cells (MPC) and cardiomyocyte progenitor cells (CPC). Initial assessment of data revealed homogeneous FPKM distribution and high inter-replicate correlation indices. Next, we identified 2208 differentially expressed (DE) lncRNA genes (log2|FC| >1; p-adjusted