GENERATION OF KNOCKOUT STEM CELL LINES TO STUDY THE FUNTIONAL ASSOCIATION OF lncRNAs AND CIS-REGULATORY REGIONS DURING CARDIAC DIFFERENTIATION

MARIA AGUSTINA SCARAFIA; ALEJANDRO DAMIÁN LA GRECA; ALAN MIQUEAS MÖBBS; MILONE, PAULA MELANIA; PÉREZ, NELBA; ARIEL WAISMAN; GUSTAVO EMILIO SEVLEVER; CARLOS DANIEL LUZZANI; LUCIA NATALIA MORO; SANTIAGO GABRIEL MIRIUKA

Virtual

Congreso; LXV Reunion Anual de Sociedades de Biociencias; 2020

Sociedad Argentina de Investigacion Clinica (SAIC)

Long non-coding RNA genes (lncRNA) were identified more than a decade ago, and since, they have been shown to fulfill a diverse range of functions. Particularly, their interaction with neighbouringregulatory regions has been proposed to modulate protein coding genes within the constraints of higher-order chromatin organizations during cell differentiation. However, many aspects of their regulatory functions are still unclear. Therefore, we set to identify lncRNAs differentially expressed (DE) during cardiac differentiation, which may be potentially involved in modulating gene expression through cis-regulatory regions (super-enhancers, SE). To this end, we first differentiated human pluripotent stem cells (hiPSC) into cardiomyocytes (CM) and performed RNA sequencing. Data analysis revealed 2,208 DE-lncRNAs between the cell populations (FDR