REGULATION OF PROLIFERATION, CELL CYCLE AND APOPTOSIS IN THE DEVELOPMENT OF RENAL TUMORS. ROLE OF PERIRENAL ADIPOSE TISSUE

FERRANDO MATÍAS; ROMEO, LEONARDO RAFAEL; GÓMEZ, SILVINA; CARRILLO, MAURO; LÓPEZ-FONTANA, CONSTANZA MATILDE; CARÓN, RUBÉN WALTER; BRUNA, FLAVIA ALEJANDRA; PISTONE-CREYDT, VIRGINIA

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias; 2023

Sociedades de Biociencia

The development of a tumor implies a deregulation of the cell cycle and apoptotic processes, and requires the interaction of tumor cells with the stromal environment. Adipose tissue is one of the most abundant stromal types. We recently demonstrated that the conditioned media (CMs) of human renal adipose tissue from patients with renal tumors (hRAT) regulates the behavior of tumor and non-tumor renal epithelial cells, differently from normal adipose tissue (hRAN). In this work, we evaluated changes in the expression of proliferation, cell cycle and apoptosis regulatory factors in: a) tumor (786-O, ACHN) and non-tumor (HK-2) human renal epithelial cell lines incubated with hRAT- or hRAN-CMs; and in b) fragments of human kidney tumors in different stages of development (different Fuhrman grade), by Western blot and immunohistochemistry.We observed a significant increase in the expression of cyclin D1 and pRB/RB in the three cell lines incubated with hRAT-CMs vs. hRAN- or control-CMs (p