Chemical composition, antioxidant, antimicrobial and antiproliferative activity of Laureliopsis philippiana essential oil of Chile, study in vitro and in silico

BRUNA, FLAVIA; FERNÁNDEZ, KATIA; URREJOLA, FELIPE; TOUMA, JORGE; NAVARRO, MYRIAM; SEPÚLVEDA, BETSABET; LARRAZABAL-FUENTES, MARÍA; PAREDES, ADRIÁN; NEIRA, IVÁN; FERRANDO, MATÍAS; OSORIO, MANUEL; YÁÑEZ, OSVALDO; BRAVO, JESSICA

Arabian Journal of Chemistry

Elsevier

Año: 2022 vol. 15 p. 1 - 28

1878-5352

Chilean Laureliopsis philippiana has been used in traditional medicine by the Mapuche and their ancestors. To evaluate its pharmacological activity, Laureliopsis philippiana leaf essential oil extract (LP_EO) was chemically and biologically characterized in the present study. In vitro antioxidant potential was analyzed, and antitumor activity was evaluated in non-tumor and tumor cell culture lines. Caenorhabditis elegans was used as a model for evaluating toxicity, and the chemical composition of the essential oil was analyzed using gas chromatography–mass spectrometry. The oil contains six major monoterpenes: eucalyptol (27.7%), linalool (27.6%), isozaphrol (19.5%), isohomogenol (12.6%), α-terpineol (7.7%), and eudesmol (4.8%). Based on quantum mechanical calculations, isosafrole and isohomogenol conferred in vitro antioxidant and antimicrobial activity to LP_EO. In addition, LP_EO showed antimicrobial activity against clinical Helicobacter pylori isolates (MIC 64 and MBC >128 μg·mL-1), Staphylococcus aureus (MIC 32 and MBC >64 μg·mL-1), Escherichia coli (MIC 8 and MBC 16 μg·mL-1) and Candida albicans (MIC 64 and >128 μg·mL-1). LP_EO could selectively inhibit the proliferation of epithelial tumor cell lines but showed low toxicity against Caenorhabditis elegans (0.39 to 1.56 μg·mL-1). Therefore, LP_EO may be used as a source of bioactive compounds in novel pharmacological treatments for veterinary and human application, cosmetics, or sanitation.