Effect of the Antioxidant Resveratrol on Photosensitized Processes

GASPAR TOSATO, MAIRA; NEYRA RECKY, JAEL R.; MIÑAN, ALEJANDRO; SCHILARDI PATRICIA ; FERNÁNDEZ LORENZO DE MELE MÓNICA ; THOMAS, ANDRÉS HÉCTOR; DANTOLA, MARIA LAURA; LORENTE, CAROLINA

Tampa, Florida

Congreso; 2018 Biennial Meeting American Society for Photobiology; 2018

AMERICAN SOCIETY FOR PHOTOBIOLOGY

Resveratrol (trans-3,5,4′-trihydroxy-stilbene; RSV) is a natural compound,1 which has been characterized as an efficient antioxidant.2 Since reactive species are always involved in photosensitized processes, including Photodynamic Inactivation (PDI) of microorganisms, the presence of antioxidants may prevent the oxidative damage. In consequence, the capacity of RSV to avoid the oxidation of biomolecules or elimination of microorganisms during these processes by RSV was evaluated. Photosensitizing processes of biomoleculesPterin (Ptr) is a natural compound that under UV-A radiation is photoactive,3 and photoinduces the degradation of biomolecules via both Type I and Type II photosensitized oxidations.4,5,6 In this work, the antioxidant capacity of RSV during Ptr photosensitized oxidation of 2´deoxyguanosine-5´monophospate (dGMP), as a model photosensitized reaction.7 Results obtained have demonstrated that RSV efficiently prevents the photodegradation of dGMP.Photodynamic inactivation of microorganismsThe elimination of planktonic Staphyloccocus aureus during PDI therapy (visible radiation and methylene blue) was evaluated in the presence of RSV. Results show that the antimicrobial efficiency of the treatment is significantly diminished in the presence of RSV, improving bacteria survival.8 1-Cucciolla V. et al, Cell Cycle 6, 2495, 20072-Quideau S. et al, Angew. Chem. Int. Ed. 50, 586, 20113-Lorente C. et. al, Acc. Chem. Res. 39, 395, 20064-Dantola M. L. et al, Pteridines 28, 105, 20175-Serrano M. et al, ChemPhysChem 16, 2244, 20156-Thomas A. H. et al, Biochimica et Biophysica Acta 1858, 139, 20167-Petroselli G. et al, J. Am. Chem. Soc. 130, 3001, 20088-Gaspar Tosato M. et al, Int. J. Antimicrob. Ag. 51, 255, 2018