Unravelling the Photoprotection Capacity of Resveratrol on Histidine Oxidation

NEYRA RECKY, JAEL R.; DANTOLA, MARIA LAURA LAURA; LORENTE, CAROLINA

Photochem

MDPI

Año: 2021

2673-7256

Exposure to sun radiation causes great oxidative stress and activates a numerous of defensemechanisms in living systems, such as the synthesis of antioxidants. Resveratrol (RSV), a naturallyoccurring stilbene molecule, has antioxidant properties and is synthesized in large amounts whenplants are under high oxidative stress. Likewise, under UV and visible radiation, biomolecules areoxidized, losing their physiological properties and, therefore, avoiding the harmful effects of solarradiation is crucial in order to preserve the functionality of cellular components. In proteins, oneessential component that is often susceptible to degradation is the amino acid histidine (His), whichcan be modified via several oxidizing mechanisms. In this article, we evaluate the photoprotectioncapacity of RSV in photosensitized oxidation of His, which is initiated with a one-electron transferreaction, yielding the His radical cation (His+). The photoprotective properties of RSV are evaluatedusing kinetics analysis during steady-state irradiation and laser flash photolysis experiments. Theexperimental results reveal that the presence of RSV in the solution causes an evident decrease ofthe His consumption initial rates as a result of a reaction between His+ and RSV that recovers theamino acid. In addition, we conclude that during its antioxidant action, RSV is consumed being asacrificial antioxidant.