Acetylcholinesterase-inhibitor hydrolysates obtained from in vitro enzymatic hydrolysis of mannoproteins extracted from different strains of yeasts

SPONTON, PABLO; SPINELLI, ROQUE; DRAGO, SILVINA; TONARELLI, GEORGINA; SIMONETTA, ARTURO

International Journal of Food Science & Technology

IFST

Lugar: Londres; Año: 2016 vol. 51 p. 300 - 308

1365-2621

In vitro inhibitory activity against acetylcholinesterase (AChE) of peptides obtained by enzymatic hydroly-sis of mannoproteins extracted from strains of yeasts was investigated. Yeast mannoproteins were extracted from strains belonging to the genera Brettanomyces, Candida, Pichia and Saccharomyces isolated from dairy products. They were obtained by heat treatment in citrate buffer and purified by affinity chro-matography with concanavalin A. Each purified extract was subsequently hydrolysed with proteolytic enzymes (trypsin, pepsin, chymotrypsin and proteinase K) applied individually or in combination, thus generating smaller peptides. Inhibitory activity of the latter against AChE was determined. The molecular weight of mannoproteins, determined by SDS-PAGE, was between 6.5 and 30 kDa. As regards AChE inhibition, a preliminary screening of all hydrolysed extracts was performed, yielding variable results with 59% maximum inhibition. Subsequently, when inhibitory concentration 50 (IC50) was determined, the extracts showed higher inhibitory activity (between 6.75 and 12.3 mg/mL mannoproteins separated from yeast strains of food origin generated bioactive peptides by enzymatic hydrolysis, which can be of interest to the manufacturing of food with potential functional properties.