In vitro effects of essential olis of Rosmarinus officinalis (romero) on Echinococcus granulosus portoscoleces

ADRIANA ANDREA ALBANESE; MARÍA CELINA ELISSONDO; LIESEL B. GENDE; MARTIN EGUARAS; GUILLERMO M. DENEGRI

Buenos Aires

Congreso; SAFE. XXXIX REUNION ANUAL DE LA SOCIEDAD AERGENTINA; 2007

SAFE

Human cystic echinoccocosis is a zoonosis caused by the metacestode Echinococcus granulosus. Today the chemotherapy of aromatic plants is becoming as alternative form of treatment. The aim of present work was determine the in vitro protoscolicidal effect of essential oils (EO) of rosmary (Rosmarinus officinalis) against E.g. Protoscoleces (PCS) were incubated with EO in water and propylenglycol (PG) as emulsifier effect at concentrations of 10, 5, 1 μg/ml. PSC incubated with culture medium alone and culture medium containing PG were used as controls. Samples of PSC from each of the dosing groups and the controls were taken every 6 days. Vitality of PSC was assessed using methylene blue exclusion technique and samples were taken for electron microscopy. Treatment finished at day 80. Vitality decreased to 9.89 ± 3.5% after 54 days. The results of vitality coincide with the tissue damage observed at the ultrastuctural level. The morphological changes included contraction of the soma region, formation of blebs on the tegument, rostellar disorganization, and loss of hooks and destruction of microtriches. Thus EO os rosmary can hopefully de considered in the future for more evaluations with other EO and synthetics drugs as benzimidazole carbamate derivates to evaluate the possible antihelmintc synergic mechanisms on PCS.