ADENOSINE REGULATES CYTOTOXIC CD4 T LYMPHOCYTES IN TRYPANOSOMA CRUZI INFECTION

BERGERO, GASTÓN; MAZZOCO YANINA; EBERHARDT, NATALIA; AOKI, MARIA PILAR

Congreso; Reunión Anual de Sociedades de Biociencias; 2021

Growing evidence demonstrates a critical role of purinergic system in the modulation of the immune response and to determine the outcome of infections. Damaged cells release the pro-inflammatory molecule ATP, which is metabolized by the ectonucleotidases CD39 and CD73 to the anti-inflammatory mediator adenosine (ADO).The mechanisms involved in the induction of CD4 T cell population with cytotoxic effector functions (CD4 CTL) have not been evaluated in the setting of experimental T. cruzi infection. Here, we explored the effect of CD73 activity abrogation (CD73KO) on the differentiation and functional capacity of the CD4 CTL population in T. cruzi infected mice.Through flow cytometry, we found that expression of multiple effector molecules (granzyme B, perforin, IFN- and TNFα) and the degranulation marker, CD107a, were increased in CD4 T spleen cells of CD73KO mice compared to WT mice at 14 days post-infection (dpi) (p