Fistulina pumiliae a new species associated with Nothofagus pumilio in Patagonia (Argentina)

BARROETAVEÑA, CAROLINA; RAJCHENBERG, MARIO; MARIA BELEN PILDAIN; GABRIELA CELESTE GONZALEZ

Santiago de Chile

Congreso; X Congreso Latinoamericano de Micología; 2021

Universidad de Chile

Fistulina Bull. is a poroid genus of wood-rooting fungi that includes annual and edible species. Nine species are currently accepted: F. hepatica, F. subhepatica B.K. Cui & J Song and F. guzmanii Brusis associated to the temperate regions of Europe, China and North and Central America, and F. spiculifera (Cooke) D.A. Reid, F. rosea Mont., F. africana Van der Byl, F. tasmanica B.K. Cui & Y.F. Sun, F. antarctica Speg. and F. endoxantha Speg. reported from the Southern Hemisphere. In Patagonia, both F. antarctica and F. endoxantha, grow on live and dead wood and stumps of native Nothofagagaceae trees, and are recognized as edible mushrooms with ancestral use by the local Mapuche ? Tehuelche communities. In this study, a detailed account of Fistulina in Patagonia (Argentina) is presented based on an integrative analysis of phylogenetic, morphological and host relationships data analysis. The survey included the collection of Fistulina specimens in the Patagonian Andes forests from Neuquén, Rio Negro and Chubut provinces in Argentina. Sequences of the internal transcribed spacer (ITS) and large subunit (LSU) were generated and analyzed. Macro and micromorphology characterization were performed, culture macro and micro-morphology were characterized and host associations were recorded. The phylogenetic analyses revealed that the Patagonian species form a strongly supported group together with species from the Southern Hemisphere. Within this group, the Patagonian collections formed three strongly supported species-level lineages: F. Antarctica lineage, along with another sequence extracted from the databases for this species; the F. endoxantha lineage in a basal position of the Southern Hemisphere cluster; and a new phylogenetic group characterized as a new species, named Fistulina pumiliae, entirely represented by Patagonian collections from Nothofagus pumilio host. The morphological characteristic that identifies the proposed new taxon are the distinguishing ochre to brown colour of its basidiomata, and the larger spore size compared to the other recorded species.