TUMORS HARVESTED FROM LSP1 KO MICE HAVE A LOWER FREQUENCY OF TOTAL LEUCOCYTES DUE TO MIGRATION DEFECTS

PASCUAL MM, DHO ND, MARIN C, ACLAND STRACK RP, CRESPO MI, PISTORESI MC, MALETTO BA, MORÓN VG

Tucuman

Congreso; LXVII REUNIÓN SAI; 2019

1. TUMORS HARVESTED FROM LSP1 KO MICE HAVE A LOWER FREQUENCY OF TOTALLEUCOCYTES DUE TO MIGRATION DEFECTSPascual MM1,2, Dho ND1,2, Marin C1,2, Acland Strack RP1,2, Crespo MI1,2, Pistoresi MC1,2, MalettoBA1,2, Morón VG1,2.1Facultad de Ciencias Químicas, UNC. 2CIBICI, CONICET-UNC.Leukocyte-specific protein 1 (LSP1) is a 52kDa cytoplasmic F-actin binding phosphoproteinexpressed in all human and murine leukocytes as well as in endothelial cells. This protein inknown as an important regulator of actin cytoskeleton remodeling. It was reported that LSP1polymorphisms or downregulation are considered risk factors for some types of cancer.We previously shown that tumors in Lsp1-/- mice grew significantly faster and bigger than inwild type (WT) controls. In order to study the role of LSP1 in antitumor immune response, weemployed the B16-OVA melanoma model. WT and Lsp1-/- mice were subcutaneously-injectedinjected with 105 B16-OVA cells and followed-up until day 15.Tumors were analyzed by flow cytometry, showing a lower frequency of total infiltratingleukocytes (CD45+ cells) in tumors obtained from Lsp1-/- mice compared to their WTcounterpart (p