MODIFICATION IN THE BIODISTRIBUTION OF THE COMPONENTS OF A VACCINE, USING AS A PLATFORM A LIQUID CRYSTAL NANOSTRUCTURE.

MARIN, CONSTANZA; RUIZ MORENO, FEDERICO N.; SÁNCHEZ VALLECILLO, MARÍA F.; CHIODETTI, ANA L.; PALMA, SANTIAGO D.; MORÓN, GABRIEL; PISTORESI-PALENCIA, MARÍA C.; ALLEMANDI, DANIEL A; MALETTO, BELKYS A.

La Plata

Taller; Taller sobre vacunas: Vacunología 2018; 2018

In the last years much effort in vaccinology has focused on the new formulation strategies for subunit vaccines. We formulated OVA (antigen) and CpG-ODN with a nanostructure formed by self-assembly of 6-O-ascorbyl palmitate (Coa-ASC16). We showed that OVA/CpG-ODN/Coa-ASC16 (G2), elicited an adaptive immune response superior to those induced by an aqueous formulation, OVA/CpG-ODN (G1). However, we do not know exactly the mechanisms of action of Coa-ASC16. Hence, this work was focused on test the impact of this nanoformulation on biodistribution of vaccine components and early immune response.Methods: mice were s.c. immunized with G1 or G2. OVA and CpG-ODN were labeled with near-infrared fluorescent dye, and were measured with Odyssey® CLx at several time points. Cytokines/chemokines were evaluated in plasma by a multiplex assay at 1.5h p.i.Results: are indicated as G1 vs G2.Liver: OVA signal showed statistically significant differences, with G1>G2 at 20 min (p